AlzMed

JP

RESEARCH

Research Achievements

List of Publications

  • Yamazaki H, Koganezawa N, Yokoo H, Sekino Y, Shirao T. “Super-resolution imaging reveals the relationship between CaMKIIβ and drebrin within dendritic spines” Neurosci. Res. In press
  • Lin W, Shiomoto S, Yamada S, Watanabe H, Kawashima Y, Eguchi Y, Muramatsu K, Sekino Y. “Dendritic spine formation and synapse maturation in transcription factor-induced human iPSC-derived neurons” iScience. 2023 Feb 27;26(4):106285. doi: 10.1016/j.isci.2023.106285. eCollection 2023 Apr 21.
  • Yamamura M, Hanamura K, Koganezawa N, Furubayashi S, Shirao T, Kawabe H. “Impacts of methotrexate on survival, dendrite development, and synapse formation of cortical neurons.” Genes Cells. 2023 May 12. doi: 10.1111/gtc.13035.
  • Xie MJ, Yagi H, Iguchi T, Yamazaki H, Hanamura K, Matsuzaki H, Shirao T, Sato M. “Phldb2 is essential for regulating hippocampal dendritic spine morphology through drebrin in an adult-type isoform-specific manner.” Neurosci Res. 2022 Dec;185:1-10. doi: 10.1016/j.neures.2022.09.010.
  • Koganezawa N, Roppongi RT, Sekino Y, Tsutsui I, Higa A, Shirao T. “Easy and Reproducible Low-Density Primary Culture using Frozen Stock of Embryonic Hippocampal Neurons.” J Vis Exp. 2023 Jan 27;(191). doi: 10.3791/64872.
  • Kobayashi Y, Kohbuchi S, Koganezawa N, Sekino Y, Shirao T , Saido TC, Saito T, Saito Y, “Impairment of ciliary dynamics in an APP knock-in mouse model of Alzheimer’s disease” Biochem Biophys Res Commun 2022 Jun 25;610:85-91. doi: 10.1016/j.bbrc.2022.04.050.
  • Togo K, Fukusumi H, Shofuda T, Ohnishi H, Yamazaki H, Hayashi MK, Kawasaki N, Takei N, Nakazawa T, Saito Y, Baba K, Hashimoto H, Sekino Y, Shirao T, Mochizuki H, Kanemura Y. Postsynaptic structure formation of human iPS cell-derived neurons takes longer than presynaptic formation during neural differentiation in vitro. Mol Brain. 2021 Oct 11;14(1):149. doi: 10.1186/s13041-021-00851-1. PMID: 34629097.
  • Kobayashi Y, Tomoshige S, Imakado K, Sekino Y, Koganezawa N, Shirao T, Diniz GB, Miyamoto T, Saito Y. Ciliary GPCR-based transcriptome as a key regulator of cilia length control. FASEB BioAdvances. 2021;00:1–24. https://doi.org/10.1096/fba.2021-00029
  • Koganezawa N, Sekino Y, Kawakami H, Fuchino H, Kawahara N, Shirao T. “NMDA receptor-dependent and -independent effects of natural compounds and crude drugs on synaptic states as revealed by drebrin imaging analysis” Eur J Neurosci. 2021 Jun;53(11):3548-3560. doi: 10.1111/ejn.15231.
  • Chaji D, Venkatesh VS, Shirao T, Day DJ, Ellenbroek BA. “Genetic Knockout of the Serotonin Reuptake Transporter Results in the Reduction of Dendritic Spines in In vitro Rat Cortical Neuronal Culture.” J Mol Neurosci. 2021 Jan 5. doi: 10.1007/s12031-020-01764-9.
  • Kobayashi Y, Okada T, Miki D, Sekino Y, Koganezawa N, Shirao T, Diniz GB, Saito Y. Properties of primary cilia in melanin-concentrating hormone receptor 1-bearing hippocampal neurons in vivo and in vitro. Neurochem Int. 2021 Jan;142:104902. doi: 10.1016/j.neuint.2020.104902.
  • Ariyani W, Miyazaki W, Amano I, Hanamura K, Shirao T, Koibuchi N. “Soy Isoflavones Accelerate Glial Cell Migration via GPER-Mediated Signal Transduction Pathway.” Front Endocrinol (Lausanne). 2020 Nov 4;11:554941. doi: 10.3389/fendo.2020.554941.
  • Yasuda H, Yamamoto H, Hanamura K, Mehruba M, Kawamata T, Morisaki H, Miyamoto M, Takada S, Shirao T, Ono Y, Mukai H. “PKN1 promotes synapse maturation by inhibiting mGluR-dependent silencing through neuronal glutamate transporter activation.” Commun Biol. 2020 Nov 26;3(1):710. doi: 10.1038/s42003-020-01435-w.
  • Dombroski TCD, Peixoto-Santos JE, Maciel K, Baqui MMA, Velasco TR, Sakamoto AC, Assirati JA, Carlotti CG, Machado HR, Sousa GK, Hanamura K, Leite JP, Costa da Costa J, Palmini AL, Paglioli E, Neder L, Spreafico R, Shirao T, Garbelli R, Martins AR.　“Drebrin expression patterns in patients with refractory temporal lobe epilepsy and hippocampal sclerosis.” Epilepsia. 2020 Jul 14. doi: 10.1111/epi.16595.
  • Koganezawa N, Shirao T. “Development of an in vitro assay to evaluated higher brain functions for drug discovery” in Columns, AMED iD3 Catalyst Unit, http://www.id3catalyst.jp/e/column09.html (2020)
  • Puspitasari A, Yamazaki H, Kawamura H, Nakano T, Takahashi A, Shirao T, Held KD. “X-irradiation of developing hippocampal neurons causes changes in neuron population phenotypes, dendritic morphology and synaptic protein expression in surviving neurons at maturity” Neurosci. Res. 2020 Nov;160:11-24. doi: 10.1016/j.neures.2019.11.002.
  • Hanamura K, Koganezawa N, Kamiyama K, Tanaka N, Oka T, Yamamura M, Sekino Y, Shirao T. “High-content imaging analysis for detecting the loss of drebrin clusters along dendrites in cultured hippocampal neurons.” J Pharmacol Toxicol Methods. 2018 Sep – Oct;99:106607. doi: 10.1016/j.vascn.2019.106607.
  • Mitsuoka T, Hanamura K, Koganezawa N, Kikura-Hanajiri R, Sekino Y, Shirao T. “Assessment of NMDA receptor inhibition of phencyclidine analogues using a high-throughput drebrin immunocytochemical assay” J Pharmacol Toxicol Methods 2019 May 10:106583. doi: 10.1016/j.vascn.2019.106583.
  • Miki D, Kobayashi Y, Okada Y, Miyamoto T, Takei N, Sekino Y, Koganezawa N, Shirao T, Saito Y　“Characterization of functional primary cilia in human induced pluripotent stem cell-derived neurons” 2019 Neurochem Res. 44(7):1736-1744. doi: 10.1007/s11064-019-02806-4.
  • Yin XL, Jia QF, Zhang GY, Zhang JP, Shirao T, Jiang CX, Yin XY, Liu YS, Chen P, Gu XC, Qian ZK, Yin GZ, Sen Xia H, Hui L. “Association between decreased serum TBIL concentration and immediate memory impairment in schizophrenia patients.” Sci Rep. 2019 Feb 7;9(1):1622. doi: 10.1038/s41598-018-38227-6.
  • Yasuda H, Kojima N, Yamazaki H, Hanamura K, Sakimura K, Shirao T. “Drebrin isoforms critically regulate NMDAR- and mGluR-dependent LTD induction” Front Cell Neurosci. 2018 Oct 8;12:330. doi: 10.3389/fncel.2018.00330. eCollection 2018.
  • Yamazaki H, Sasagawa Y, Yamamoto H, Bito H, Shirao T. “CaMKIIβ is localized in dendritic spines as both drebrin-dependent and drebrin-independent pools” J. Neurochem. (2018) 146:145-159. doi: 10.1111/jnc.14449.
  • Hanamura K, Kamata Y, Yamazaki H, Kojima N, Shirao T, “Isoform-dependent regulation of drebrin dynamics in dendritic spines.” Neuroscience (2018) 379: 67–76. Doi 10.1016/j.neuroscience.2018.02.038
  • Miao S, Koganezawa N, Hanamura K, Puspitasari A, Shirao T. “N-methyl-D-aspartate receptor mediates X-irradiation-induced drebrin decrease in hippocampus” Kitakanto Medical Journal (2018) 68: 111-115.
  • Yamazaki H, Shirao T.　“Homer, Spikar, and Other Drebrin-Binding Proteins in the Brain”Adv Exp Med Biol. 2017;1006:249-268. doi: 10.1007/978-4-431-56550-5_14
  • Sekino Y, Koganezawa N, Mizui T, Shirao T. “Role of Drebrin in Synaptic Plasticity” Adv Exp Med Biol. 2017;1006:183-201. doi: 10.1007/978-4-431-56550-5_11.
  • Shirao T, Koganezawa N, Yamazaki H, Hanamura K, Imamura K. “Localization of Drebrin: Light Microscopy Study” Adv Exp Med Biol. 2017;1006:105-118. doi: 10.1007/978-4-431-56550-5_7
  • Shirao T, Sekino Y. “General Introduction to Drebrin” Adv Exp Med Biol. 2017;1006:3-22. doi: 10.1007/978-4-431-56550-5_1.
  • Shirao T., Sekino Y. (Eds.) (2017) Drebrin. (Advances in Experimental Medicine and Biology, vol 1006) Tokyo, Springer
  • Li B, Ding S, Feng N, Mooney N, Ooi YS, Ren L, Diep J, Kelly MR, Yasukawa LL, Patton JT, Yamazaki H, Shirao T, Jackson PK, Greenberg HB “Drebrin restricts rotavirus entry by inhibiting dynamin-mediated endocytosis” Proc Natl Acad Sci U S A. 2017 May 2;114(18):E3642-E3651. doi: 10.1073/pnas.1619266114. Epub 2017 Apr 17.
  • Kajita Y, Kojima N, Koganezawa N, Yamazaki H, Sakimura K, Shirao T. “Drebrin E regulates neuroblast proliferation and chain migration in the adult brain “ Eur J Neurosci. 2017 Sep;46(6):2214-2228. doi: 10.1111/ejn.13668. Epub 2017 Sep 7.
  • Koganezawa N, Hanamura K, Sekino Y, Shirao T. “The role of drebrin in dendritic spines” Mol Cell Neurosci. 2017 Feb 1. pii: S1044-7431(16)30205-6. doi: 10.1016/j.mcn.2017.01.004. [Epub ahead of print] Review.
  • Shirao T, Hanamura K, Koganezawa N, Ishizuka Y, Yamazaki H, Sekino Y. “The role of drebrin in neurons” J Neurochem. 2017 Jun;141(6):819-834. doi: 10.1111/jnc.13988. Epub 2017 Apr 21. Review.
  • Kojima K, Yasuda H, Hanamura K, Ishizuka Y, Sekino Y, Shirao T. “Drebrin A regulates hippocampal LTP and hippocampus-dependent fear learning in adult mice” Neuroscience. 324: 218–226 (2016)　 10.1016/j.neuroscience.2016.03.015
  • Puspitasari A, Koganezawa N, Kojima N., Tanaka N, Nakano T, Shirao T “X-irradiation induces acute cognitive decline via transient synaptic dysfunction.” Rad. Res. 185, 423–430 (2016) 10.1667/RR14236.1
  • Shirao T., Koganezawa N. (2015) The Role of Drebrin-Binding Stable Actin Filaments in Dendritic Spine Morphogenesis. pp.363-372, In: Schatten H. (eds) The Cytoskeleton in Health and Disease. Springer, New York, NY
  • Ohara Y, Koganezawa N, Yamazaki H, Roppongi RT, Sato K, Sekino Y, Shirao T, “Early-stage development of human-induced pluripotent stem cell (hiPSC)-derived neurons” J. Neurosci. Res. 93:1804-13 (2015)　 10.1002/jnr.23666
  • Shioda N, Sawai M, Ishizuka Y, Shirao T., Fukunaga K. “Nuclear translocation of calcium/calmodulin-dependent protein kinase IIδ3 promoted by protein phosphatase-1 enhances brain-derived neurotrophic factor expression in dopaminergic neurons.” J. Biol. Chem. 290:21663-21675 (2015) 10.1074/jbc.M115.664920
  • Shimizu H, Ishizuka Y, Yamazaki H, Shirao T. “Allopregnanolone increases mature excitatory synapses along dendrites via protein kinase A signaling” Neuroscience 305:139-45 (2015) 10.1016/j.neuroscience.2016.03.015
  • Shi-Qiong Xu, Simone Buraschi, Alaide Morcavallo, Marco Genua, Tomoaki Shirao, Stephen C. Peiper, Leonard G. Gomella, Ruth Birbe, Antonino Belfiore, Renato V. Iozzo and Andrea Morrione “A novel role for drebrin in regulating progranulin bioactivity in bladder cancer.” Oncotarget 6:10825-10839 (2015) 10.18632/oncotarget.3424
  • Shimizu H, Ishizuka Y, YamazFujieda T, Koganezawa N, Ide Y, Shirao T and Sekino Y. “A Inhibitory Pathway Controlling the Gating Mechanism of the Lateral Amygdala Revealed by Voltage Sensitive Dye Imaging” Neuroscience Letters, 590:126-131(2015)
  • Kudo S, Suzuki Y, Noda S, Mizui T, Shirai K, Okamoto M, Kaminuma T, Yoshida Y, Shirao T and Nakano T. “Comparison of the radiosensitivities of neurons and glial cells derived from the same rat brain.” Exp. Ther. Med. 8: 754-758, 2014
  • Yuta Ishizuka, Hideo Shimizu, Eiko Takagi, Mai Kato, Hirotaka Yamagata, Masahiko Mikuni, Tomoaki Shirao. “Histone deacetylase mediates the decrease in drebrin cluster density induced by amyloid beta oligomers” Neurochem. Int. 76:114-121 (2014)
  • Tanabe K, Yamazaki H, Inaguma Y, Asada A, Kimura T, Takahashi J, Taoka M, Ohshima T, Furuichi T, Isobe T, Nagata K, Shirao T, Hisanaga S. “Phosphorylation of drebrin by cyclin-dependent kinase 5 and its role in neuronal migration.” PLoS One. 9:e92291 (2014)
  • Ishikawa M, Shiota J, Ishibashi1 Y, Hakamata T, Shoji S, Fukuchi M, Masaaki Tsuda M, Shirao T, Sekino Y, Baraban JM and Tabuchi A. “Cellular localization and dendritic function of rat isoforms of the SRF coactivator MKL1 in cortical neurons.” NeuroReport 25:585-592 (2014)
  • Mizui T, Sekino Y, Yamazaki1, Yuta Ishizuka H, Takahashi H, Kojima N, Kojima M and ShiraoT. “Myosin II ATPase activity mediates the long-term potentiation-induced exodus of stable F-actin bound by drebrin A from dendritic spines” PLoS One. 9(1):e85367. (2014)
  • Yamazaki H, Kojima N, Kato K, Hirose H, Iwasaki T, Mizui T, Takahashi H, Hanamura K, Roppongi RT, Koibuchi N, Sekino Y, Mori N, Shirao T. “Spikar, a novel drebrin-binding protein, regulates the formation and stabilization of dendritic spines.” J Neurochem. 128: 507-522 (2014)
  • Ishikawa M, Shiota J, Ishibashi Y, Hakamata T, Shoji S, Fukuchi M, Tsuda M, Shirao T, Sekino Y, Ohtsuka T, Baraban JM and Tabuchi A. “Identification, expression and characterization of rat isoforms of the SRF coactivator MKL1” FEBS Open Bio. 3:387-93 (2013)
  • Yuta Ishizuka, Naomasa Kakiya, Lee A. Witters, Noriko Oshiro, Tomoaki Shirao, Hiroyuki Nawa and Nobuyuki Takei “AMP-activated protein kinase (AMPK) counteracts brain-derived neurotrophic factor (BDNF)-induced mammalian target of rapamycin complex 1 (mTORC1) signaling in neurons” J Neurochem. 127:66-77 (2013)
  • Shirao T, González-Billault C “Actin filaments and microtubules in dendritic spines.” J Neurochem 126: 155-164 (2013)
  • Roppongi RT, Kojima N, Hanamura K, Yamazaki H, Shirao T. “Selective reduction of drebrin and actin in dendritic spines of hippocampal neurons by activation of 5-HT2A receptors.” Neurosci. Lett. 547:76-81 (2013)
  • Shirai K, Mizui T, Suzuki Y, Okamoto M, Hanamura K, Yoshida Y, Hino M, Noda S, Al-HahdariI, Chakravarti A, Shirao T, Nakano T, “X-Irradiation Changes Dendritic Spine Morphology and Density through Reduction of Cytoskeletal Proteins in Mature Neurons” Radiation Research 179: 630-636 (2013)
  • K Kato, T Shirao, H Yamazaki, K Imamura, Y Sekino: Regulation of AMPA receptor recruitment by the actin binding protein drebrin in cultured hippocampal neurons. J. Neurosci. Neuroeng. 1: 153-160 (2012)
  • Okamoto, T, Shirao, T, Shutoh, F, Suzuki, T,  Nagao, S“Post-training cerebellar cortical activity plays an important role for consolidation of memory of cerebellum-dependent motor learning.” Neurosci Lett. 504: 53-56. (2011)
  • Annalisa Mancini, Dario Sirabella, Weijia Zhang, Hiroyuki Yamazaki, Tomoaki Shirao and Robert S Krauss “Regulation of myoblast fusion by the actin-binding factor drebrin” Skeletal Muscle 1-36 (2011)
  • Kobayashi-Yamazaki C, Shirao T, Sasagawa Y, Maruyama Y, Akita H, Saji M, and Sekino Y. “Lesions of the supramammillary nucleus decrease self-grooming behavior of rats placed in an open field” The Kitakanto Medical Jorunal , 61：287-292 (2011)
  • Tanaka K, Sato K, Yoshida T, Fukuda T, Hanamura K, Kojima N, Shirao T, Yanagawa T, and Watanabe H. “Evidence for cell density affecting C2C12 myogenesis: possible regulation of myogenesis by cell-cell communication.” Muscle and Nerve, 44:968-977 (2011)
  • Han W, Takamatsu Y, Yamamoto H, Kasai S, Endo S, Shirao T, Kojima N, Ikeda K. “Inhibitory role of inducible cAMP early repressor (ICER) in methamphetamine-induced locomotor sensitization” PLoS One 6(6):e21637 (2011)
  • Okamoto T,, Endo S, Shirao T, and Nagao S. “Role of Cerebellar Cortical Protein Synthesis in Transfer of Memory Trace of Cerebellum-Dependent Motor Learning” J. Neurosci. 31: 8958-8966 (2011)
  • Kaminuma T, Suzuki Y, Shirai K, Mizui T, Noda S, Yoshida Y, Funayama T, Takahasi T, Kobayashi Y, Shirao T, Nakano T. “Effectiveness of carbon-ion beams for apoptosis induction in rat primary immature hippocampal neurons” J Radiat Res (Tokyo)  51:627-631 (2010)
  • Hanamura, K, Mizui T, Kakizaki T, Roppongi TR, Yamazaki H, Yanagawa Y, Shirao T. “Low accumulation of drebrin at glutamatergic postsynaptic sites on GABAergic neurons” Neuroscience 169: 1489-1500 (2010)
  • Pérez-Martínez M, Gordón-Alonso M, Cabrero JR, Barrero-Villar M, Rey M, Mittelbrunn M, Lamana A, Morlino G, Calabia C, Yamazaki H, Shirao T, Vázquez J, González-Amaro R, Veiga E, Sánchez-Madrid F. “F-actin-binding protein drebrin regulates CXCR4 recruitment to the immune synapse” J. Cell Sci. 123:1160-1170 (2010)
  • Mercer JC, Mottram LF Qi Q, Lee YC, Bruce D, Iyer A, Yamazaki H, Shirao T, Choe MH,. Peterson BR, August A “Chemico-Genetic Identification of Drebrin as a Regulator of Calcium Responses.” Int. J. Biochem. Cell Biol. 42:337-345 (2010)
  • Kojima N, Hanamura K, Yamazaki H, Ikeda T, Itohara S, Shirao T. “Genetic disruption of the alternative splicing of drebrin gene impairs context-dependent fear learning in adulthood” Neuroscience 165: 138-150 (2010)
  • Okamoto M, Suzuki Y, Shirai K, Mizui T, Yoshida Y, Noda S,. Al-Jahdari WS, Shirao T, Nakano T. “Effect of Irradiation on the Development of Immature Hippocampal Neurons In Vitro” Radiat Res 172:718-724 (2009)
  • Aoki C, Kojima N, Sabaliauskas N, Shah L, Oakford J, Ahmed T, Yamazaki H, Hanamura K, Shirao T “Drebrin A Knock-Out Eliminates the Rapid Form of Homeostatic Synaptic Plasticity at Excitatory Synapses of Intact Adult Cerebral Cortex” J Comp Neurol  517:105-121 (2009)
  • Ito M, Shirao T, Doya K, Sekino Y. “Three-dimensional distribution of Fos-positive neurons in the supramammillary nucleus of the rat exposed to novel environment.” Neurosci. Res. 64: 379-402 (2009)
  • Mizui T., Kojima N, Yamazaki H, Katayama M, Hanamura K, Shirao T. “Drebrin E is involved in the mechanism regulating axonal growth through actin-myosin interactions.” J Neurochem 109:611-622 (2009).
  • Takahashi, T, Yamazaki, H, Hanamura K, Sekino Y and Shirao T “AMPA receptor inhibition causes abnormal dendritic spines by destabilizing drebrin” J Cell Sci. 122:1211-1229 (2009)
  • Ivanov A, Esclapez M, Pellegrino1 C, Shirao T, and Ferhat L. “Drebrin A regulates the dendritic spine plasticity and synaptic function in cultured hippocampal neurons” J. Cell Sci. 122: 524-534 (2009)
  • Song M, Kojima N, Hanamura K, Sekino Y, Inoue KH, Mikuni M, Shirao T, “Expression of drebrin E in migrating neuroblasts in adult rat brain: coincidence between drebrin E disappearance from cell body and cessation of migration” Neuroscience. 152:670-682 (2008)
  • Aoki C, Mahadomrongkul V, Fujisawa S, Habersat R, Shirao T. “Chemical and morphological alterations of spines within the hippocampus and entorhinal cortex precedes the onset of Alzheimer’s disease pathology in double knock-in mice” J. Comp. Neurol. 505:352–362 (2007)
  • Sekino Y, Shirao T. “Role for signal propagation through the hippocampal CA2 field in memory formation”, pp254-266. in Web Intelligence Meets Brain Informatics Ning Zhong, Jiming Liu, Yiyu Yao, Jinglong Wu, Shengfu Lu, Kuncheng Li (Eds) in Lecture Notes in Artificial Intelligence. Springer (2007) (book)
  • Sekino Y, Kojima N, Shirao T. “Role of actin cytoskeleton in dendritic spine morphogenesis” Neurochem. Int. 51: 92-104 (2007)
  • Kobayashi, C., Aoki, C., Kojima, N., Yamazaki, H., and Shirao, T., “Drebrin A content correlates with spine head size in the adult mouse cerebral cortex” J. Comp. Neurol. 503:618-626 (2007)
  • Kato, K., Sekino, Y., Takahashi, H., Yasuda, H., Shirao, T., “Increase of AMPA receptors-mediated miniature EPSC amplitude after chronic NMDA receptor blockade in cultured hippocampal neurons” Neurosci. Lett. 418:4-8 (2007)
  • Kojima, N., Shirao, T. “Synaptic dysfunction and disruption of the postsynaptic drebrin-actin complex: the study of neurological disorders accompanied by cognitive deficits.” Neurosci. Res. 58:1-5 (2007)
  • Majoul, I., Shirao, T., Sekino, Y., Duden, R. “Many faces of Drebrin: from building dendritic spines and stabilizing gap junctions to shaping neurite-like cell processes” Histochem. Cell Biol. 127:355-361 (2007)
  • Shirai, K., Mizui, T., Suzuki, Y., Kobayashi, Y., Nakano, T., Shirao, T. “Differential effects of x-irradiation on immature and mature hippocampal neurons in vitro.” Neurosci. Lett. 399: 57-60 (2006)
  • Chang EH, Savage MJ, Flood DG, Thomas JM, Levy RB, Mahadomrongkul, V., Shirao, T., Aoki, C., Huerta, P.T. “AMPA receptor downscaling at the onset of Alzheimer’s pathology in double knock-in mice” Proc. Natl. Acad. Sci. (USA) 103: 3410-3415 (2006)
  • Fujisawa, S., Shirao, T., Aoki, C. “In vivo, competitive blockade of NMDA receptors induces rapid shape change of post-synaptic spines and F-actin reorganization within dendritic spines of adult rat cortex.” Neuroscience 140:1177-1187 (2006)
  • Takahashi, H., Mizui T. and Shirao, T. “Downregulation of drebrin A expression suppresses synaptic targeting of NMDA receptors in developing hippocampal neurons” J. Neuochem  97(s1):110-115 (2006)
  • Sekino, Y., Tanaka, S., Hanamura, K., Yamazaki, H., Sasagawa, Y., Xue Y., Hayashi, K., and Shirao, T. “Activation of N-methyl-D-aspartate receptor induces a shift of drebrin distribution: disappearance from dendritic spines and appearance in dendritic shafts” Mol. Cell. Neurosci. 31: 493-504 (2006)
  • Mahadomrongkul, V., Huerta, P.T., Shirao, T., Aoki, C. “Stability of the distribution of spines containing drebrin A in the sensory cortex layer I of mice expressing mutated APP and PS1 genes” Brain Res. 1064: 66-74 (2005)
  • Mizui, T., Takahashi, H., Sekino, Y., and Shirao, T. “Overexpression of drebrin A in immature neurons induces the accumulation of F-actin and PSD-95 into dendritic filopodia, and the formation of large abnormal protrusions.” Mol. Cell. Neurosci. 30: 149 – 157 (2005)
  • Aoki, C., Sekino, Y., Hanamura, K., Fujisawa, S., Mahadomrongkul, V., Ren, Y., and Shirao, T. “Drebrin A is a Postsynaptic Protein that Localizes in vivo to the Submembranous Surface of Dendritic Sites Forming Excitatory Synapses” J. Comp. Neurol. 483: 383-402 . (2005)
  • Hanaoka, R., Ohmori Y., Uyemura, K., Hosoya, T., Hotta, Y., Shirao, T., and Okamoto, H .”Zebrafish gcmb is required for pharyngeal cartilage formation” Mech Dev. 121:1235-1247 (2004)
  • Butkevich E, Huelsmann S, Wenzel D, Shirao T, Duden R., and Majoul I., “Drebrin is a novel Connexin-43 binding partner that links gap junctions to the submembrane cytoskeleton.” Curr. Biol. 14:650-658 (2004)
  • Kobayashi R, Sekino Y, Shirao T, Tanaka S, Ogura T, Inada K, and Saji M. “Antisense knockdown of drebrin A, a dendritic spine protein, causes stronger preference, impaired pre-pulse inhibition, and an increased sensitivity to psychostimulant.” Neurosci. Res.49:205-217 (2004)
  • Tezuka M, Oda O, Shirao T, Inoue HK., “Microglia/macrophage reactions and cell proliferation during the repair of spinal cord injuries in infant rats” Neurotrauma Res, 15: 17-20 (2003)
  • Takahashi H., Sekino Y, Tanaka S, Mizui T, Kishi S, Shirao T, “Drebrin-dependent Actin Clustering in Dendritic Filopodia Governs Synaptic Targeting of Postsynaptic Density-95 and Dendritic Spine Morphogenesis” J. Neurosci. 23:6586–6595 (2003)
  • Ferhat, L., Esclapez, M., Represa, A., Fattoum, A., Shirao ,T., and Ben-Ari, Y. “Upregulation of acidic calponin during dendritic spine plasticity following pilocarpine-induced seizures” Hippocampus 13:845-858 (2003)
  • Jin, Mi., Tanaka,S., Sekino,Y., Ren,Y., Yamazaki,H., Kawai-Hirai,R., Kojima,N. and Shirao,T.　“A Novel Brain-Specific Mouse Drebrin: cDNA Cloning, Chromosomal Mapping, Genomic Structure, Expression, and Functional Characterization” Genomics 79:686-692 (2002)
  • Oda,O., Tezuka, M., Nichi, H., Nishimatsu, T., Kobayashi, S., Shirao, T., Inoue, HK., “Cellular responsesand cavity formation after brain injury in infant and adult rats” Neurotrauma Res, 13 11-15 (2001)
  • Tezuka, M., Oda, O., Nishi, H., Nagaseki, Y., Nishimatsu, T., Kobayashi, S., Shirao, T., Inoue, HK, “Vimentin-expressing astorcytes and dendrite regeneration during spinal cord repair in infant rats.” Neurotrauma Res, 13: 59-62 (2001)
  • Tomidokoro,Y., Harigaya,Y., Matsubara,E., Ikeda,M., Kawarabayashi,T., Shirao,T., Ishiguro,K., Okamoto,K., Yonkin,S.G. and Shoji,M. “ Brain Aß amyloidosis in APPsw mice induces accumulation of presenilin-I and tau.” J. Pathol. 193: 500-506 (2001)
  • Kobayashi,S., Shirao,T., and Sasaki,T. “Drebrin expression is increased in spinal motoneurons after axotomy.” Neuorsci. Lett. 311: 165-168 (2001)
  • Ikeda, M., Segara,M., Sekino,Y., Shirao,T., Honda,K., Yoshioka,T., Allen,CN. and Inoue,S.“The sulphydryl reagent, N-ethylmaleimide, disrupts sleep and blocks A1 adenosine receptor-mediated inhibition of intracellular calcium signaling in the in vitro ventromedial preoptic nucleus.” Neurosci. 106: 733-743 (2001)
  • Yamazaki,H., Takahashi,H., Aoki,T. and Shirao,T. “Molecular Cloning and Dendritic Localization of rat SH3P7” Eur. J. Neurosci. 14: 998-1008 (2001)
  • Shirao,T. and Sekino,Y. “Clustering and anchoring mechanisms of molecular constituents of postsynaptic scaffolds in the spine” Neurosci. Res. 40: 1-7 (2001)
  • Tezuka,M., Oda,O., Nishi,H., Nagaseki,Y., Nishimatsu,T., Kobayashi,S., Shirao,T. and Inoue,H.K. “Cellular and molecular changes during the process of repair of spinal cord injury in infant rats.” Neurotrauma Res. 12：57-60 (2000)
  • Tanaka,S., Sekino,Y. and Shirao,T. “The effects of neurotrophin-3 and brain-derived neurotrophic factor on cerebellar granule cell movement and neurite extension in vitro.” Neurosci. 97: 727-734 (2000)
  • Cheng,X-T., Hayashi,K. and Shirao,T. “Non-muslcle myosin IIB-like immunoreactivity is present at the drebrin –binding cytoskeleton in neurons” Neurosci. Res. 36: 167-173 (2000).
  • Hayashi,K., Ishikawa,R., Kawai-Hirai,R., Takagi,T., Taketomi,A. and Shirao,T. “Domaoin ananlysis of the actin-binding and actin-remodeling activities of drebrin.” Exp. Cell Res. 253: 673-680 (1999)
  • Hatanpaa,K., Isaacs,K.R., Shirao,T., Brady,DR. and Rapoport,S.I.” Aging of the human brain: Loss of synaptic proteins regulating plasticity.” J. Neuropathol. and Exp. Neurol. 58: 637-643 (1999)
  • Ochiishi,T., Saitoh,Y., Yukawa,A., Saji,M., Ren,Y., Shirao,T., Miyamoto,H., Nkakta,H. and Sekino,Y. “High level of adenosine A1 receptor-like immunoreactivity in the CA2/CA3a region of the adult rat hippocampus” Neurosci. 93:955-967(1999)
  • Hayashi ,K.and Shirao,T. “Change in the shape of dendritic spines caused by overexpression of drebrin in cultured cortical neurons” J. Neurosci. 19: 3918-3925 (1999)
  • Toda,M., Shirao,T. and Uyemura,K. “Suppression of a actin-binding prtoein, drebrin, by antisense transfection inhibits neurite outgrowth in neuroblastoma B104 cells” Devl. Brain Res. 114: 193-200 (1999)
  • Hayashi,K., and Shirao,T. “Visualization of dendritic spine formation with GFP-fusion proteins.” in Neural Devbelopment, K Uyemura eds. Springer-Verlag, Tokyo, pp. 399-403 (1999)
  • Shirao,T., Yong,R., Hayashi,K., and Sekino,Y. “Time-lapse video microscopic analysis of cell process formation from drebrin A expressing fibroblast.” in Neural Devbelopment, Uyemura,K., Kawamura,K., Yazaki,T. eds. Springer-Verlag, Tokyo, pp. 381-385 (1999)
  • Shirao,T. “Drebrin” in Guidebook to the Cytoskeletal and Motor Proteins . T Kreis and R Vale eds. Oxoford University Press, N Y pp.76-77 (1999)
  • Shimohama,S., Fujimoto,S., Sumida,Y., Akagawa,K., Shirao,T., Matsuoka,Y. and Taniguchi,T. “Differential expression of rat brain synaptic prtoeins in development and aging.” Biochem. Biophys. Res. Comm. 251：394-398 (1998)
  • Hayashi,K., Suzuki,K., and Shirao,T. “ Rapid conversion of drebrin isoforms during synapse formation in primary culture of cortical neurons.” Devel. Brain Res. 111: 137-141 (1998)
  • Mammoto,A., Sasaki,T., Asakura,T., Hotta,I., Imamura,H., Takahashi,K., Matsura,Y., Shirao,T., Takai,Y. “Interactions of drebrin and gephyrin with profilin.” Biochem. Biophys. Res. Commun. 243: 86-89 (1998)
  • 31 Inoue,H.K., and Shriao,T. “Neurite formation induced in neuroblastoma cells and genetically altered nonneuronal cells.” J. Electron Microscopy 46: 497-502(1997)
  • 30 Hayashi,K., Ishikawa,R., Li-Hong,Y., HE,X., Takata,K., Kohama,K. and Shirao,T. “Modulatory role of drebrin on the cytoskeleton within dendritic spines in rat cerebral cortex.” J. Neurosci. 15: 7161-7170 (1996)
  • 29 Sasaki,Y., Hayashi,K., Shirao,T., Ishikawa,R. and Kohama,K “Inhibition by drebrin of the actin-binding activity of brain fascin, a protein localized in filopodia of growth cones.” J. Neurochem.66: 980-988 (1996)
  • 28 Ikeda,K., Kaub,P.A., Asada,H., Uyemura,K., Toya,S. and Shirao,T. “Stabilization of adhesion plaques by the expression of drebrin A in fibroblasts.” Dev.Brain Res. 91: 227-236 (1996)
  • 27  Harigaya, Y., Shoji,M., Shirao,T., and Hirai,S. “Disappearance of actin-binding protein, drebrin, from hippocampal sysnapses in Alzheimer’s Disease.” J. Neurosci. Res. 43: 87-92 (1996)
  • 26 Kobayashi,S., Isa,K., Hayashi,K., Inoue,H.K., Uyemura,K. and Shirao,T. “K252a, a potent inhibitor of protein kinases, inhibits the migration of cerebellar granule cells in vitro” Dev. Brain Res. 90: 122-128 (1995)
  • 25 Nakatsubo,N., Arai,O. and Shirao,T. “Identification of the newly generated neruons of canaries during adulthood” Dokyo Journal of Medical Sciences 22, 65-72 (1995)
  • 24 Ikeda,K., Shirao,T., Toda,M., Asada,H., Toya,S. and Uyemura,K. “Effect of neuron-specific actin-binding protein, drebrin A, on cell-substratum adhesion.” Neurosci. Lett. 194, 197-200 (1995)
  • 23 Shirao,T., “The roles of microfilament-associated proteins, drebrins in brain morphogenesis: A Review.” J. Biochem. 117, 231-236 (1995)
  • 22 Ishikawa,R., Hayashi,K., Shirao,T., Xue,Y., Takagi,T., Sasaki,Y. and Kohama,K. “Drebrin, a development-associated brain protein from rat embryo, causes the dissociation of tropomyosin from actin filaments” J. Biol. Chem. 269:29928-29933 (1994)
  • 21 Shirao,T., Hayashi,K., Ishikawa,R., Isa,K., Asada,H., Ikeda,K. and Uyemura,K. “Formation of thick curving bundles of actin by drebrin A expressed in fibroblasts.” Exp. Cell Res. 215:145-153 (1994)
  • 20 Asada,H., Uyemura,K. and Shirao,T. “Actin-binding protein, drebrin, accumulates in submembranous regions in parallel with neuronal differentiation.” J. Neurosci. Res. 38: 149-159 (1994)
  • 19 Toda,M., Shirao,T., Minoshima,S., Shimizu,N., Toya,S. and Uyemura,K.”Molecular cloning of cDNA encoding the actin-binding protein, human drebrin E and Chromosomal mapping of drebrin gene #.” Biochem.Biophys.Res.Commu. 196: 468-472 (1993)
  • 18 Kojima,N., Shirao,T. and Obata,K “Molecular cloning of a developmentally regulated brain protein chicken drebrin A and its expression by alternative splicing of the drebrin gene” Mol. Brain Res.19: 101-114 (1993)
  • 17 Inoue, H.K.,Nakamura,M. and Shirao,T “Process of neurite formation and genetic engineering.” J. Neural Transplantation and Plasticity. 3：287 (1992)
  • 16 Obata, K.,Shirao ,T.,and Kojima,N. “Drebrins, developmentally regulated proteins in the chick nervous system, are similarly expressed in the mammals” pp. 46-49. in Molecular Basis of Neuronal Connectivity. (Edited by Satake,M., Obata,K., Hatanaka,H., Miyamoto,E and Okuyama,T. Kohko-do) (1992)
  • 15 Kaub,P., Shirao,T., Inoue,A., Takeda, Y., Uyemura,K. “Identification of rat drebrin embryonic isoform by polymerase chain reaction and its genomic analysis.” Bulletin Jap. Neurochem. Soc. 31: 148-149 (1992)
  • 14 Shirao,T., Evinger, M.J., Iacovitti ,L and Reis,D.J. “Lesions of nigrostriatal pathway reduce expression of tyrosine hydroxylase gene in residual dopaminergic neurons of substantia nigra.” Neurosci. Lett. 141: 208-212 (1992)
  • 13 Imamura, K.,Shirao,T., Mori ,K.and Obata,K. “Changes of drebrin expression in the visual cortex of the cat during development.” Neurosci.Res 13: 33-41 (1992)
  • 12 Shirao,T., Kojima,N. and Obata,K “Cloning of drebrin A and induction of neurite-like processes in drebrin-transfected cells.” NeuroReport 3: 109-112 (1992)
  • 11 Shirao,T., Kojima,N., Terada,S. and Obata,K. “Expression of three drebrin isoforms in the developing nervous system.”  Neurosci. Res. S13: S106-S111 (1990)
  • Shirao,T.,Kojima,N., Nabeta,Y., and Obata,K. “Two forms of drebrins, developmentally regulated brain proteins, in rat.”  Proc. Japan Acad 65: 169-172 (1989)
  • Kojima,N., Kato,Y., Shirao,T. and Obata,K “Nucleotide sequences of two embryonic drebrins, developmentally regulated brain proteins, and developmental change in their mRNAs.”  Mol. Brain Res 4: 207-215 (1988)
  • Shirao,T., Kojima,N., Kato,Y. and Obata,K. “Molecular cloning of a cDNA for the developmentally regulated brain protein, drebrin.” Mol.Brain Res. 4: 71-74 (1988)
  • Kohama,K., Mitani,S. and Shirao,T. “Effect of N-(N-(L-trans-3-carboxyoxirane-2-carbonyl)-L- leucyl)-3-methyl-butylamine (E64C), a thiolprotease inhibitor, on tyrosine release from skeletal muscle cells.”  Proc. Japan Acad. 63: 376-380 (1987 )
  • Obata,K., Kojima,N., Nishiye,H., Inoue,H., Shirao,T .,Fujita,S.C. and Uchizono,K. “Four synaptic vesicle-specific proteins: identification by monoclonal antibodies and distribution in the nervous tissue and the adrenal medulla.”  Brain Res 404: 169-179. (1987)
  • Obata,K., Shirao,T., Kojima,N. and Tanaka,H “Molecular events associated with neural development.” Acta Neurochirugica Suppl. 41: 3-7 (1987)
  • Shirao,T., Inoue,H.K., Kano,Y. and Obata,K. “Localization of a developmentally regulated neuron-specific protein S54 in dendrites as revealed by immunoelectron microscopy.” Brain Res. 413: 374-378 (1987)
  • Shirao,T and Obata,K. “Immunochemical homology of 3 developmentally regulated brain proteins and their developmental change in neuronal distribution.” Dev. Brain Res. 29: 233-244 (1986)
  • Obata,K ., Nishiye,H., Fujita,S.C.,  Shirao,T., Inoue,H and Uchizono,K “Identification of a synaptic vesicle-specific 38,000-dalton protein by monoclonal antibodies.” Brain Res 375: 37-48 (1986)
  • Shirao,T and Obata,K.”Two acidic proteins associated with brain development in chick embryo.” J. Neurochem. 44: 1210-1216. (1985)